#Waters acquity uplc sample manager full#

Result:A total of 10 quality differential markers were searched from raw products, roasted products and honey-roasted products of G. Differential components were identified according to the relative molecular weight, fragment ion, mass spectrum database and related literature information, then the migration of components before and after processing was studied. The data set collected after pretreatment was analyzed with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) to quickly search the differential components in different processed products of G. uralensis dry roots and rhizomes before and after processing by negative ion mode. Method:Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MSE) was used to collect high-precision mass-charge ratio and ion response strength information of the components in G. Objective:To identify the quality differential markers of different processed products of Glycyrrhiza uralensis dry roots and rhizomes. Conclusion:The established UPLC-Q-TOF/MSE can comprehensively and rapidly analyze the chemical constituents in Jiechangyan Qixiao granulesпјЊ and preliminarily elucidates the chemical composition profile of this granulesпјЊ which can lay a foundation for further research on the pharmacodynamic material basis and quality control of Jiechangyan Qixiao granules. Among the identified chemical constituentsпјЊ there were 28 from Drynariae RhizomaпјЊ 31 from Plantaginis SemenпјЊ 53 from Chebulae Fructus and 58 ingredients from Crataegi Fructus. Result:A total of 102 chemical components were separated and identified in Jiechangyan Qixiao granulesпјЊ including organic acidsпјЊ flavonoids and its glycosidesпјЊ triterpenesпјЊ phenylethanoid glycosidesпјЊ tanninsпјЊ iridoid glycosides and other componentsпјЊ among which flavonoids and its glycosides were from Drynariae Rhizoma and Crataegi FructusпјЊ phenylethanoid glycosides and iridoid glycosides were from Plantaginis SemenпјЊ triterpenoids and tannins were from Crataegi Fructus and Chebulae Fructus. The ion peaks were identified by comparing with the information of control substancesпјЊ literature references and self-built database. MSE mode was used to collect mass spectral data. Electrospray ionization пј€ESIпј‰ was applied for scanning under positive and negative ion modes with the scanning range of m/z 60-1 200. min-1пјЊ the column temperature was 30 в„ѓпјЊ and the volume of sample injection was 2 ОјL.Sample carryover: Wash solvents: Two degassed: strong solvent and weak wash solvent, programmable to suit application.Minimum sample required: 5 uL residual, using maximum recovery 2-mL vials (zero offset).Sample probe: XYZZ based needle-in-needle design.30 sec with single weak wash, lO-iJL loop, pressure assist mode.

At an ambient temperature of 21.0 в™ј or lower the sample manager will maintain the temperature of the sample compartment down to 4.0 в™ј with a tolerance of -2.0/+6.0 в™ј, when configured with the maximum number of vials and/or plates Sample temperature control:0 to 40.0 в™ј, settable in 0.1 в™ј increments (assumes an ambient temperature of 25.0 в™ј).Sample delivery precision(full loop injection mode): 0.999 coefficient of deviation (from 20% to 75%, partial loop overfill mode (PLNO injection mode), per Waters' AQT/SystemsQT protocol.

#Waters acquity uplc sample manager full#

Injection volume range: 0.1 to 50.0 iJL, in 0.1 iJL increments, partial or full loop mode 10ul loop is standard 1, 2, 5, 20, and 50-uL loops also available.Number of sample injections: 1 to 99 injections per sample.Maximum sample capacity: 768 in two 384-well plates expandable to up to 8,448 samples with optional Sample Organizer.

24 position 1.50-mL micro-centrifuge tube plates.

48 position 0.65-mL micro-centrifuge tube plates.

Number of sample plates: Total of two plates, expandable to up to 22 plates with optional Sample Organizer (see below):.